| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KCNN4 (C-Term) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
KCNN4 |
| Gene ID: |
3783 |
| Uniprot ID: |
KCNN4_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
Specific for KCNN4. |
| Immunogen: |
A synthetic peptide from the c-terminal region of human KCNN4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Post Translational Modifications | Phosphorylation at His-358 by NDKB activates the intermediate conductance calcium-activated potassium channel activity, and conversely it's dephosphorylation by PHPT1 inhibits this activity. |
| Function | Intermediate conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening. The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 25 picosiemens. Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV. Controls calcium influx during vascular contractility by being responsible of membrane hyperpolarization induced by vasoactive factors in proliferative vascular smooth muscle cell types. Following calcium influx, the consecutive activation of KCNN4 channel leads to a hyperpolarization of the cell membrane potential and hence an increase of the electrical driving force for further calcium influx promoting sustained calcium entry in response to stimulation with chemotactic peptides. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis through activation by PI(3)P. |
| Protein Name | Intermediate Conductance Calcium-Activated Potassium Channel Protein 4Skca 4Skca4Hsk4Gardos ChannelIkca1Hik1Kca3.1Putative Gardos ChannelHkca4 |
| Database Links | Reactome: R-HSA-1296052 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionRuffle MembraneTargeted To Membrane Ruffles After Egf Stimulation |
| Alternative Antibody Names | Anti-Intermediate Conductance Calcium-Activated Potassium Channel Protein 4 antibodyAnti-Skca 4 antibodyAnti-Skca4 antibodyAnti-Hsk4 antibodyAnti-Gardos Channel antibodyAnti-Ikca1 antibodyAnti-Hik1 antibodyAnti-Kca3.1 antibodyAnti-Putative Gardos Channel antibodyAnti-Hkca4 antibodyAnti-KCNN4 antibodyAnti-IK1 antibodyAnti-IKCA1 antibodyAnti-KCA4 antibodyAnti-SK4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
