| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
KCNK2 |
| Gene ID: |
3776 |
| Uniprot ID: |
KCNK2_HUMAN |
| Immunogen Region: |
297-411 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 297-411 of human KCNK2 (NP_055032.1). |
| Immunogen Sequence: |
RVISKKTKEEVGEFRAHAAE WTANVTAEFKETRRRLSVEI YDKFQRATSIKRKLSAELAG NHNQELTPCRRTLSVNHLTS ERDVLPPLLKTESIYLNGLT PHCAGEEIAVIENIK |
| Tissue Specificity | Isoform 3: Detected in kidney, adrenal gland and brain where it is preferentially expressed in the amygdala but not found in thalamus, hypothalamus, hippocampus or substantia nigra. |
| Post Translational Modifications | Phosphorylation at Ser-348 controls the reversible conversion from a leak channel to a voltage-dependent channel. |
| Function | K(+) channel that conducts voltage-dependent outward rectifying currents upon membrane depolarization. Voltage sensing is coupled to K(+) electrochemical gradient in an 'ion flux gating' mode where outward but not inward ion flow opens the gate. Converts to voltage-independent 'leak' conductance mode upon stimulation by various stimuli including mechanical membrane stretch, acidic pH, heat and lipids. Reversibly converts between a voltage-insensitive K(+) 'leak' channel and a voltage-dependent outward rectifying K(+) channel in a phosphorylation-dependent manner. Homo- and heterodimerizes to form functional channels with distinct regulatory and gating properties. In trigeminal ganglia sensory neurons, the heterodimer of KCNK2/TREK-1 and KCNK18/TRESK inhibits neuronal firing and neurogenic inflammation by stabilizing the resting membrane potential at K(+) equilibrium potential as well as by regulating the threshold of action potentials and the spike frequency. At trigeminal A-beta afferent nerves, the heterodimer of KCNK2/TREK-1 and KCNK4/TRAAK is mostly coexpressed at nodes of Ranvier where it conducts voltage-independent mechanosensitive and thermosensitive currents, allowing rapid action potential repolarization, high speed and high frequence saltatory conduction on myelinated nerves to ensure prompt sensory responses. In hippocampal astrocytes, the heterodimer of KCNK2/TREK-1 and KCNK1/TWIK-1 allows passive K(+) conductance under basal conditions, but changes ion selectivity and becomes permeable to L-glutamate and Cl(-) ions upon binding to G-protein subunit GNG4 in stimulated astrocytes. Mediates rapid L-glutamate release in response to activation of G-protein-coupled receptors, such as F2R and CNR1. In hippocampal pyramidal neurons, the homodimer of KCNK2/TREK-1 contributes to gamma-aminobutyric acid (GABA) B-induced slow inhibitory postsynaptic potential. Associates with AKAP5 and Gs-protein-coupled receptor B2AR at postsynaptic dense bodies and converts to a leak channel no longer sensitive to stimulation by arachidonic acid, acidic pH or mechanical stress, nor inhibited by Gq-coupled receptors but still under the negative control of Gs-coupled receptors. Permeable to other monovalent cations such as Rb(+) and Cs(+). Isoform 4: Does not display channel activity but reduces the channel activity of isoform 1 and isoform 2 and reduces cell surface expression of isoform 2. |
| Protein Name | Potassium Channel Subfamily K Member 2Outward Rectifying Potassium Channel Protein Trek-1Trek-1 K(+ Channel SubunitTwo Pore Domain Potassium Channel Trek1Two Pore Potassium Channel Tpkc1K2p2.1 |
| Database Links | Reactome: R-HSA-1299503Reactome: R-HSA-5576886 |
| Cellular Localisation | Isoform 2: Cell MembraneMulti-Pass Membrane ProteinIsoform 3: Cell MembraneIsoform 4: Endoplasmic Reticulum MembraneCell ProjectionAxonDendritePostsynaptic Density MembraneCell MembraneSarcolemmaLocalizes At The Ranvier Nodes Of Myelinated Afferent Nerves |
| Alternative Antibody Names | Anti-Potassium Channel Subfamily K Member 2 antibodyAnti-Outward Rectifying Potassium Channel Protein Trek-1 antibodyAnti-Trek-1 K(+ Channel Subunit antibodyAnti-Two Pore Domain Potassium Channel Trek1 antibodyAnti-Two Pore Potassium Channel Tpkc1 antibodyAnti-K2p2.1 antibodyAnti-KCNK2 antibodyAnti-TREK antibodyAnti-TREK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
