| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KCNK1 (290-336 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
KCNK1 |
| Gene ID: |
3775 |
| Uniprot ID: |
KCNK1_HUMAN |
| Immunogen Region: |
290-336 aa |
| Specificity: |
Specific for KCNK1. |
| Immunogen: |
A synthetic peptide from aa region 290-336 of human KCNK1 (TWIK-1) conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | Sumoylation is controversial. Sumoylated by UBE2I. Not sumoylated when expressed in xenopus oocytes or mammalian cells. Sumoylation inactivates the channel, but does not interfere with expression at the cell membrane. Sumoylation of a single subunit is sufficient to silence the dimeric channel. Sumoylation of KCNK1 is sufficient to silence heterodimeric channels formed by KCNK1 and KCNK3 or KCNK9. Desumoylated by SENP1.this activates the channel. Desumoylated by SENP1.this strongly increases halothane-mediated activation of heterodimeric channels formed with KCNK9. SENP1 treatment has no effect. |
| Function | Ion channel that contributes to passive transmembrane potassium transport and to the regulation of the resting membrane potential in brain astrocytes, but also in kidney and in other tissues. Forms dimeric channels through which potassium ions pass in accordance with their electrochemical gradient. The channel is selective for K(+) ions at physiological potassium concentrations and at neutral pH, but becomes permeable to Na(+) at subphysiological K(+) levels and upon acidification of the extracellular medium. The homodimer has very low potassium channel activity, when expressed in heterologous systems, and can function as weakly inward rectifying potassium channel. Channel activity is modulated by activation of serotonin receptors. Heterodimeric channels containing KCNK1 and KCNK2 have much higher activity, and may represent the predominant form in astrocytes. Heterodimeric channels containing KCNK1 and KCNK3 or KCNK9 have much higher activity. Heterodimeric channels formed by KCNK1 and KCNK9 may contribute to halothane-sensitive currents. Mediates outward rectifying potassium currents in dentate gyrus granule cells and contributes to the regulation of their resting membrane potential. Contributes to the regulation of action potential firing in dentate gyrus granule cells and down-regulates their intrinsic excitability. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1. Required for normal ion and water transport in the kidney. Contributes to the regulation of the resting membrane potential of pancreatic beta cells. The low channel activity of homodimeric KCNK1 may be due to sumoylation. The low channel activity may be due to rapid internalization from the cell membrane and retention in recycling endosomes. Permeable to monovalent cations with ion selectivity for K(+) > Rb(+) >> NH4(+) >> Cs(+) = Na(+) = Li(+). |
| Protein Name | Potassium Channel Subfamily K Member 1Inward Rectifying Potassium Channel Protein Twik-1Potassium Channel K2p1Potassium Channel Kcno1 |
| Database Links | Reactome: R-HSA-1299308Reactome: R-HSA-5576886 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinRecycling EndosomeSynaptic Cell MembraneCytoplasmic VesiclePerikaryonCell ProjectionDendriteApical Cell MembraneThe Heterodimer With Kcnk2 Is Detected At The Astrocyte Cell MembraneNot Detected At The Astrocyte Cell Membrane When Kcnk2 Is AbsentDetected On Neuronal Cell BodiesAnd To A Lesser Degree On Neuronal Cell ProjectionsDetected On Hippocampus Dentate Gyrus Granule Cell Bodies And To A Lesser Degree On Proximal DendritesDetected At The Apical Cell Membrane In Stria Vascularis In The CochleaDetected At The Apical Cell Membrane Of Vestibular Dark Cells Situated Between The Crista And The Utricle In The Inner EarDetected At The Apical Cell Membrane In Kidney Proximal Tubule Segment S1 And In Subapical Compartments In Segments S1S2 And S3Predominantly In Cytoplasmic Structures In Kidney Distal Convoluted Tubules And Collecting DuctsDetected At The Apical Cell Membrane Of Bronchial Epithelial Cells |
| Alternative Antibody Names | Anti-Potassium Channel Subfamily K Member 1 antibodyAnti-Inward Rectifying Potassium Channel Protein Twik-1 antibodyAnti-Potassium Channel K2p1 antibodyAnti-Potassium Channel Kcno1 antibodyAnti-KCNK1 antibodyAnti-HOHO1 antibodyAnti-KCNO1 antibodyAnti-TWIK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
