Western blot analysis of extracts of various cell lines, using KCNJ8 antibody (STJ112580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-KCNJ8 antibody (325-424) (STJ112580)

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STJ112580
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCNJ8
Gene ID: 3764
Uniprot ID: KCNJ8_HUMAN
Immunogen Region: 325-424
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 325-424 of human KCNJ8 (NP_004973.1).
Immunogen Sequence: VSIVTEEEGVYSVDYSKFGN TVKVAAPRCSARELDEKPSI LIQTLQKSELSHQNSLRKRN SMRRNNSMRRNNSIRRNNSS LMVPKVQFMTPEGNQNTSES
Tissue Specificity Predominantly detected in fetal and adult heart.
Function This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium.as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
Protein Name Atp-Sensitive Inward Rectifier Potassium Channel 8
Inward Rectifier K(+ Channel Kir6.1
Potassium Channel - Inwardly Rectifying Subfamily J Member 8
Ukatp-1
Database Links Reactome: R-HSA-1296025
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp-Sensitive Inward Rectifier Potassium Channel 8 antibody
Anti-Inward Rectifier K(+ Channel Kir6.1 antibody
Anti-Potassium Channel - Inwardly Rectifying Subfamily J Member 8 antibody
Anti-Ukatp-1 antibody
Anti-KCNJ8 antibody

Information sourced from Uniprot.org

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