• STJ71822 (0. 3µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71822 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71822 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71822 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-ITM2B antibody (Internal) (STJ71822)

SKU:
STJ71822-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ITM2B (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
IHC-2.5µg/ml
IF-Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ITM2B
Gene ID: 9445
Uniprot ID: ITM2B_HUMAN
Immunogen Region: Internal
Accession Number: NP_068839.1
Immunogen Sequence: HDKETYKLQRRETIK
Post Translational Modifications The ectodomain C-terminal part of the imBRI2 is processed by furin producing a secreted Bri23 peptide and a mature BRI2, membrane form (mBRI2). The remaining part of the ectodomain of mBRI2 containing the BRICHOS domain is cleaved by ADAM10 and is secreted (BRI2C, soluble form). The membrane-bound N-terminal fragment (BRI2C, membrane form) is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted C-peptide and a BRI2 intracellular domain (BRI2 ICD) released in the cytosol. Shedding by ADAM10 facilitates intramembrane cleavage but is not absolutely required for BRI2 ICD generation. Glycosylation at Asn-170 is important for cell surface localization, but doesn't affect furin- and ADAM10-induced proteolytic processing.
Function Plays a regulatory role in the processing of the amyloid-beta A4 precursor protein (APP) and acts as an inhibitor of the amyloid-beta peptide aggregation and fibrils deposition. Plays a role in the induction of neurite outgrowth. Functions as a protease inhibitor by blocking access of secretases to APP cleavage sites. Mature BRI2 (mBRI2) functions as a modulator of the amyloid-beta A4 precursor protein (APP) processing leading to a strong reduction in the secretion of secretase-processed amyloid-beta protein 40 and amyloid-beta protein 42. Bri23 peptide prevents aggregation of APP amyloid-beta protein 42 into toxic oligomers.
Protein Name Integral Membrane Protein 2b
Immature Bri2
Imbri2
Protein E25b
Transmembrane Protein Bri
Bri Cleaved Into - Bri2 - Membrane Form
Mature Bri2
Mbri2 - Bri2 Intracellular Domain
Bri2 Icd - Bri2c - Soluble Form - Bri23 Peptide
Bri2-23
Abri23
C-Terminal Peptide
P23 Peptide
Database Links Reactome: R-HSA-977225
Cellular Localisation Integral Membrane Protein 2b: Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Immature Bri2 (Imbri2) Is Cleaved By Furin In The Golgi Into Mbri2 And A Bri23 Peptide
Mbri2 Is Transported To The Plasma Membrane And Bri23 Peptide Is Secreted
Bri2
Membrane Form: Cell Membrane
Endosome Membrane
Mature Bri2 (Mbri2) Needs To Be Transported From The Endoplasmic Reticulum Compartment To The Cell Membrane In Order To Be Able To Inhibit App Processing
Bri23 Peptide: Secreted
Detected In The Cerebral Spinal Fluid (Csf)
Bri2c
Soluble Form: Secreted
Alternative Antibody Names Anti-Integral Membrane Protein 2b antibody
Anti-Immature Bri2 antibody
Anti-Imbri2 antibody
Anti-Protein E25b antibody
Anti-Transmembrane Protein Bri antibody
Anti-Bri Cleaved Into - Bri2 - Membrane Form antibody
Anti-Mature Bri2 antibody
Anti-Mbri2 - Bri2 Intracellular Domain antibody
Anti-Bri2 Icd - Bri2c - Soluble Form - Bri23 Peptide antibody
Anti-Bri2-23 antibody
Anti-Abri23 antibody
Anti-C-Terminal Peptide antibody
Anti-P23 Peptide antibody
Anti-ITM2B antibody
Anti-BRI antibody
Anti-BRI2 antibody

Information sourced from Uniprot.org

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