| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:20-1:50ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IREB2 |
| Gene ID: | 3658 |
| Uniprot ID: | IREB2_HUMAN |
| Immunogen Region: | 760-963 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 760-963 of human IRP2 (NP_004127.1). |
| Immunogen Sequence: | SIARNSAAAKYLTNRGLTPR EFNSYGARRGNDAVMTRGTF ANIKLFNKFIGKPAPKTIHF PSGQTLDVFEAAELYQKEGI PLIILAGKKYGSGNSRDWAA KGPYLLGVKAVLAESYEKIH KDHLIGIGIAPLQFLPGENA DSLGLSGRETFSLTFPEELS PGITLNIQTSTGKVFSVIAS FEDDVEITLYKHGGLLNFVA RKFS |
| Post Translational Modifications | Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity. |
| Function | RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. |
| Protein Name | Iron-Responsive Element-Binding Protein 2Ire-Bp 2Iron Regulatory Protein 2Irp2 |
| Database Links | Reactome: R-HSA-917937 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Iron-Responsive Element-Binding Protein 2 antibodyAnti-Ire-Bp 2 antibodyAnti-Iron Regulatory Protein 2 antibodyAnti-Irp2 antibodyAnti-IREB2 antibody |
Information sourced from Uniprot.org