| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IRAK4 |
| Gene ID: | 51135 |
| Uniprot ID: | IRAK4_HUMAN |
| Immunogen Region: | 1-460 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IRAK4 (NP_057207.2). |
| Immunogen Sequence: | MNKPITPSTYVRCLNVGLIR KLSDFIDPQEGWKKLAVAIK KPSGDDRYNQFHIRRFEALL QTGKSPTSELLFDWGTTNCT VGDLVDLLIQNEFFAPASLL LPDAVPKTANTLPSKEAITV QQKQMPFCDKDRTLMTPVQN LEQSYMPPDSSSPENKSLEV SDTRFHSFSFYELKNVTNNF DERPISVGGNKMGEGGFGVV YKGYVNNTTVAVKKLAAMVD |
| Post Translational Modifications | Phosphorylated. |
| Function | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. |
| Protein Name | Interleukin-1 Receptor-Associated Kinase 4 Irak-4 Renal Carcinoma Antigen Ny-Ren-64 |
| Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-166058 Reactome: R-HSA-5603037 Reactome: R-HSA-5603041 Reactome: R-HSA-6811558 Reactome: R-HSA-9020702 Reactome: R-HSA-975110 Reactome: R-HSA-975138 Reactome: R-HSA-975155 Reactome: R-HSA-975871 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Interleukin-1 Receptor-Associated Kinase 4 antibody Anti-Irak-4 antibody Anti-Renal Carcinoma Antigen Ny-Ren-64 antibody Anti-IRAK4 antibody |
Information sourced from Uniprot.org