• Immunofluorescence analysis of NIH/3T3 cells using IRAK4 Rabbit polyclonal antibody (STJ27964) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of IRAK4 in paraffin-embedded Human hepatobiliary duct using IRAK4 Rabbit polyclonal antibody (STJ27964) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IRAK4 in paraffin-embedded human lung cancer using IRAK4 Rabbit polyclonal antibody (STJ27964) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IRAK4 in paraffin-embedded rat heart using IRAK4 Rabbit polyclonal antibody (STJ27964) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IRAK4 in paraffin-embedded rat brain using IRAK4 Rabbit polyclonal antibody (STJ27964) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat lung, using IRAK4 Rabbit polyclonal antibody (STJ27964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-IRAK4 antibody (1-460) (STJ27964)

SKU:
STJ27964

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IRAK4 (1-460) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRAK4
Gene ID: 51135
Uniprot ID: IRAK4_HUMAN
Immunogen Region: 1-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human IRAK4 (NP_057207.2).
Immunogen Sequence: MNKPITPSTYVRCLNVGLIR KLSDFIDPQEGWKKLAVAIK KPSGDDRYNQFHIRRFEALL QTGKSPTSELLFDWGTTNCT VGDLVDLLIQNEFFAPASLL LPDAVPKTANTLPSKEAITV QQKQMPFCDKDRTLMTPVQN LEQSYMPPDSSSPENKSLEV SDTRFHSFSFYELKNVTNNF DERPISVGGNKMGEGGFGVV YKGYVNNTTVAVKKLAAMVD
Post Translational Modifications Phosphorylated.
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Protein Name Interleukin-1 Receptor-Associated Kinase 4
Irak-4
Renal Carcinoma Antigen Ny-Ren-64
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-166058
Reactome: R-HSA-5603037
Reactome: R-HSA-5603041
Reactome: R-HSA-6811558
Reactome: R-HSA-9020702
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975155
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Interleukin-1 Receptor-Associated Kinase 4 antibody
Anti-Irak-4 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-64 antibody
Anti-IRAK4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance