• Immunohistochemistry analysis of paraffin-embedded human esophagus using INTS5 antibody (STJ115605) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using INTS5 antibody (STJ115605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-INTS5 antibody (120-400) (STJ115605)

SKU:
STJ115605

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-INTS5 (120-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INTS5
Gene ID: 80789
Uniprot ID: INT5_HUMAN
Immunogen Region: 120-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human INTS5 (NP_085131.1).
Immunogen Sequence: DVVQEVQQVLSEFIRANPKA WAPVISAWSIDLMGQLSSTY SGQHQRVPHATGALNELLQL WMGCRATRTLMDIYVQCLSA LIGSCPDACVDALLDTSVQH SPHFDWVVAHIGSSFPGTII SRVLSCGLKDFCVHGGAGGG AGSSGGSSSQTPSTDPFPGS PAIPAEKRVPKIASVVGILG HLASRHGDSIRRELLRMFHD SLAGGSGGRSGDPSLQATVP FLLQLAVMSPALLGTVSGE
Function Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.
Protein Name Integrator Complex Subunit 5
Int5
Database Links Reactome: R-HSA-6807505
Cellular Localisation Nucleus Membrane
Multi-Pass Membrane Protein
Nucleus
Cytoplasm
Alternative Antibody Names Anti-Integrator Complex Subunit 5 antibody
Anti-Int5 antibody
Anti-INTS5 antibody
Anti-KIAA1698 antibody

Information sourced from Uniprot.org

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