• Confocal immunofluorescence analysis of U2OS cells using IMMT Rabbit polyclonal antibody (STJ24199) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of IMMT in paraffin-embedded rat heart tissue using IMMT Rabbit polyclonal antibody (STJ24199) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Mouse brain, using IMMT Rabbit polyclonal antibody (STJ24199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using IMMT Rabbit polyclonal antibody (STJ24199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IMMT antibody (120-270) (STJ24199)

SKU:
STJ24199

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IMMT (120-270) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IMMT
Gene ID: 10989
Uniprot ID: MIC60_HUMAN
Immunogen Region: 120-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-270 of human IMMT (NP_006830.2).
Immunogen Sequence: ESKQPASQLQKQKGDTPASA TAPTEAAQIISAAGDTLSVP APAVQPEESLKTDHPEIGEG KPTPALSEEASSSSIRERPP EEVAARLAQQEKQEQVKIES LAKSLEDALRQTASVTLQAI AAQNAAVQAVNAHSNILKAA MDNSEIAGEKK
Function Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology.
Protein Name Micos Complex Subunit Mic60
Cell Proliferation-Inducing Gene 4/52 Protein
Mitochondrial Inner Membrane Protein
Mitofilin
P87/89
Database Links Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Mitochondrion
Alternative Antibody Names Anti-Micos Complex Subunit Mic60 antibody
Anti-Cell Proliferation-Inducing Gene 4/52 Protein antibody
Anti-Mitochondrial Inner Membrane Protein antibody
Anti-Mitofilin antibody
Anti-P87/89 antibody
Anti-IMMT antibody
Anti-HMP antibody
Anti-MIC60 antibody
Anti-MINOS2 antibody
Anti-PIG4 antibody
Anti-PIG52 antibody

Information sourced from Uniprot.org

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