| Host: |
Goat |
| Applications: |
Pep-ELISA |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Il6ra-Mouse (Internal) is suitable for use in ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments in Mouse Spleen and Thymus lysates and in lysates of cell lines NIH3T3 and NSO gave no specific signal but low background (at antibody concentration up to 1µg/ml |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_034689.2 |
| Specificity: |
This antibody is expected to recognize all reported isoforms (NP_000556.1; NP_852004.1). The sequence of the immunizing peptide corresponds with a part of the extracellular domain. |
| Immunogen Sequence: |
YLLQFQLRYRPVWSK |
| Post Translational Modifications | A short soluble form is also released from the membrane by proteolysis. The sIL6R is formed by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding. mIL6R is cleaved by the proteases ADAM10 and ADAM17. Glycosylated. Glycosylation is dispensable for transport, signaling, and cell-surface turnover. Glycosylation at Asn-55 is a protease-regulatory exosite. Glycosylation is required for ADAM17-mediated proteolysis. |
| Function | Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells. Interleukin-6 receptor subunit alpha: Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Soluble interleukin-6 receptor subunit alpha: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the proinflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. |
| Protein Name | Interleukin-6 Receptor Subunit AlphaIl-6 Receptor Subunit AlphaIl-6r Subunit AlphaIl-6r-AlphaIl-6raIl-6r 1Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit AlphaSil6r |
| Database Links | Reactome: R-MMU-1059683Reactome: -MMU-110056Reactome: -MMU-112411 |
| Cellular Localisation | Interleukin-6 Receptor Subunit Alpha: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Interleukin-6 Receptor Subunit Alpha: Secreted |
| Alternative Antibody Names | Anti-Interleukin-6 Receptor Subunit Alpha antibodyAnti-Il-6 Receptor Subunit Alpha antibodyAnti-Il-6r Subunit Alpha antibodyAnti-Il-6r-Alpha antibodyAnti-Il-6ra antibodyAnti-Il-6r 1 antibodyAnti-Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha antibodyAnti-Sil6r antibodyAnti-Il6ra antibodyAnti-Il6r antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
