• Western blot analysis of extracts of various cell lines, using CD25 antibody (STJ24183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD25 rabbit polyclonal antibody (STJ24183) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using CD25 rabbit polyclonal antibody (STJ24183) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat spleen cells using CD25 rabbit polyclonal antibody (STJ24183) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Flow cytometry: 293F Transfection cells (+) and 293F cells (-) were stained with rabbit IgG isotype control (10 Mu g/mL, blue line) or CD25 rabbit polyclonal antibody (STJ24183, 2. 5 Mu g/mL orange line) , followed by Goat anti-rabbit polyclonal antibody Alexa Fluor 647 (1:600 dilution) staining. Non-fluorescently stained Daudi cells was used as blank control (red line).

Anti-IL2RA antibody (153-167) (STJ24183)

SKU:
STJ24183

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/FC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL2RA (153-167) is suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL2RA
Gene ID: 3559
Uniprot ID: IL2RA_HUMAN
Immunogen Region: 153-167
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 153-167 of human CD25 (NP_000408.1).
Immunogen Sequence: VQGYRALHRGPAESV
Function Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Protein Name Interleukin-2 Receptor Subunit Alpha
Il-2 Receptor Subunit Alpha
Il-2-Ra
Il-2r Subunit Alpha
Il2-Ra
Tac Antigen
P55
Cd Antigen Cd25
Database Links Reactome: R-HSA-5673001
Reactome: R-HSA-8877330
Reactome: R-HSA-9020558
Reactome: R-HSA-912526
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interleukin-2 Receptor Subunit Alpha antibody
Anti-Il-2 Receptor Subunit Alpha antibody
Anti-Il-2-Ra antibody
Anti-Il-2r Subunit Alpha antibody
Anti-Il2-Ra antibody
Anti-Tac Antigen antibody
Anti-P55 antibody
Anti-Cd Antigen Cd25 antibody
Anti-IL2RA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance