• Western blot analysis of extracts of various cell lines, using Il1b antibody (STJ11103361) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of RAW264. 7 cells, using Il1b antibody (STJ11103361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Il1b rabbit polyclonal antibody (STJ11103361) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spleen using Il1b rabbit polyclonal antibody (STJ11103361) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using Il1b rabbit polyclonal antibody (STJ11103361) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human tonsil using Il1b rabbit polyclonal antibody (STJ11103361) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Il1b antibody (118-269) (STJ11103361)

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STJ11103361

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Il1b (118-269) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Il1b
Gene ID: 16176
Uniprot ID: IL1B_MOUSE
Immunogen Region: 118-269
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 118-269 of mouse IL1 Beta (NP_032387.1).
Immunogen Sequence: VPIRQLHYRLRDEQQKSLVL SDPYELKALHLNGQNINQQV IFSMSFVQGEPSNDKIPVAL GLKGKNLYLSCVMKDGTPTL QLESVDPKQYPKKKMEKRFV FNKIEVKSKVEFESAEFPNW YISTSQAEHKPVFLGNNSGQ DIIDFTMESVSS
Tissue Specificity Expressed in activated macrophages (at protein level).
Function Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Protein Name Interleukin-1 Beta
Il-1 Beta
Database Links Reactome: R-MMU-448706
Reactome: -MMU-5620971
Reactome: -MMU-5660668
Reactome: -MMU-9020702
Cellular Localisation Cytoplasm
Cytosol
Secreted
Lysosome
Extracellular Exosome
The Precursor Is Cytosolic
In Response To Inflammasome-Activating Signals
Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome
Cleaved And Secreted
Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore
In Contrast
The Precursor Form Is Not Released
Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
Alternative Antibody Names Anti-Interleukin-1 Beta antibody
Anti-Il-1 Beta antibody
Anti-Il1b antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance