• STJ71925 (0. 1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71925 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71925 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi and cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71925 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-IL18 antibody (Internal) (STJ71925)

SKU:
STJ71925-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-IL18 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: IL18
Gene ID: 3606
Uniprot ID: IL18_HUMAN
Immunogen Region: Internal
Accession Number: NP_001553.1; NP_001230140.1
Specificity: This antibody is expected to recognise both reported isoforms (NP_001553.1; NP_001230140.1).
Immunogen Sequence: NPPDNIKDTKSDI
Function Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Protein Name Interleukin-18
Il-18
Iboctadekin
Interferon Gamma-Inducing Factor
Ifn-Gamma-Inducing Factor
Interleukin-1 Gamma
Il-1 Gamma
Database Links Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-9012546 Q14116-2
Reactome: R-HSA-9660826
Cellular Localisation Cytoplasm
Secreted
The Precursor Is Cytosolic
In Response To Inflammasome-Activating Signals
Cleaved And Secreted
Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore
In Contrast
The Precursor Form Is Not Released
Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
Alternative Antibody Names Anti-Interleukin-18 antibody
Anti-Il-18 antibody
Anti-Iboctadekin antibody
Anti-Interferon Gamma-Inducing Factor antibody
Anti-Ifn-Gamma-Inducing Factor antibody
Anti-Interleukin-1 Gamma antibody
Anti-Il-1 Gamma antibody
Anti-IL18 antibody
Anti-IGIF antibody
Anti-IL1F4 antibody

Information sourced from Uniprot.org

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