• Immunofluorescence analysis of U-2 OS cells using IL13RA2 Rabbit polyclonal antibody (STJ11103023) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using IL13RA2 Rabbit polyclonal antibody (STJ11103023) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using IL13RA2 Rabbit polyclonal antibody (STJ11103023) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat liver, using IL13RA2 Rabbit polyclonal antibody (STJ11103023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using IL13RA2 Rabbit polyclonal antibody (STJ11103023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IL13RA2 antibody (50-150) (STJ11103023)

SKU:
STJ11103023

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL13RA2
Gene ID: 3598
Uniprot ID: I13R2_HUMAN
Immunogen Region: 50-150
Specificity: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IL13RA2 (NP_000631.1).
Immunogen Sequence: YLQWQPPLSLDHFKECTVEY ELKYRNIGSETWKTIITKNL HYKDGFDLNKGIEAKIHTLL PWQCTNGSEVQSSWAETTYW ISPQGIPETKVQDMDCVYYN W
Post Translational Modifications Cleaved by MMP8 leading to a soluble form that is also able to interact with IL13.
Function Cell surface receptor that plays a role in the regulation of IL-13-mediated responses. Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation. Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1.
Protein Name Interleukin-13 Receptor Subunit Alpha-2
Il-13 Receptor Subunit Alpha-2
Il-13r Subunit Alpha-2
Il-13r-Alpha-2
Il-13ra2
Interleukin-13-Binding Protein
Cd Antigen Cd213a2
Database Links Reactome: R-HSA-6785807
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interleukin-13 Receptor Subunit Alpha-2 antibody
Anti-Il-13 Receptor Subunit Alpha-2 antibody
Anti-Il-13r Subunit Alpha-2 antibody
Anti-Il-13r-Alpha-2 antibody
Anti-Il-13ra2 antibody
Anti-Interleukin-13-Binding Protein antibody
Anti-Cd Antigen Cd213a2 antibody
Anti-IL13RA2 antibody
Anti-IL13R antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance