| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IL10RA (265-578) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IL10RA |
| Gene ID: |
3587 |
| Uniprot ID: |
I10R1_HUMAN |
| Immunogen Region: |
265-578 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 265-578 of human IL10RA. (NP_001549.2). |
| Immunogen Sequence: |
KLPSVLLFKKPSPFIFISQR PSPETQDTIHPLDEEAFLKV SPELKNLDLHGSTDSGFGST KPSLQTEEPQFLLPDPHPQA DRTLGNREPPVLGDSCSSGS SNSTDSGICLQEPSLSPSTG PTWEQQVGSNSRGQDDSGID LVQNSE |
| Tissue Specificity | Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells. |
| Post Translational Modifications | Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation. Ubiquitinated by BTRC.ubiquitination leads to endocytosis and subsequent degradation of IL10RA. |
| Function | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy. |
| Protein Name | Interleukin-10 Receptor Subunit AlphaIl-10 Receptor Subunit AlphaIl-10r Subunit AlphaIl-10raCdw210aInterleukin-10 Receptor Subunit 1Il-10r Subunit 1Il-10r1Cd Antigen Cd210 |
| Database Links | Reactome: R-HSA-6783783 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasm |
| Alternative Antibody Names | Anti-Interleukin-10 Receptor Subunit Alpha antibodyAnti-Il-10 Receptor Subunit Alpha antibodyAnti-Il-10r Subunit Alpha antibodyAnti-Il-10ra antibodyAnti-Cdw210a antibodyAnti-Interleukin-10 Receptor Subunit 1 antibodyAnti-Il-10r Subunit 1 antibodyAnti-Il-10r1 antibodyAnti-Cd Antigen Cd210 antibodyAnti-IL10RA antibodyAnti-IL10R antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
