Anti-IL10 antibody (STJ140130)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant human IL10 produced in E. coli.
General Information
| Short Description | Goat polyclonal Interleukin 10 antibody for use in WB, IHC-P and IHC-F in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Datasheet included with dilution |
| Applications | WB/IHC-P/IHC-F |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 3 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:250-1:5000IHC-F 1:250-1:1000IHC-P 1:250-1:1000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | IL10 |
| Gene ID | 3586 |
| Uniprot ID | IL10_HUMAN |
| Accession Number | ENSG00000136634 |
| Immunogen | Purified recombinant human IL10 produced in E. coli. |
| Immunogen Sequence | MHSSALLCCLVLLTGVRASP HSSALLCCLVLLTGVRASPG SSALLCCLVLLTGVRASPGQ SALLCCLVLLTGVRASPGQG ALLCCLVLLTGVRASPGQGT LLCCLVLLTGVRASPGQGTQ LCCLVLLTGVRASPGQGTQS CCLVLLTGVRASPGQGTQSE CLVLLTGVRASPGQGTQSEN |
| Specificity | Detects endogenous levels of total IL10 by Western blot in whole cell and tissue lysates. |
Additional Info
| Tissue Specificity | Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. |
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. |
| Protein Name | Interleukin-10Il-10Cytokine Synthesis Inhibitory FactorCsif |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-8950505Reactome: R-HSA-9662834Reactome: R-HSA-9664323Reactome: R-HSA-9725370 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-10 antibodyAnti-Il-10 antibodyAnti-Cytokine Synthesis Inhibitory Factor antibodyAnti-Csif antibodyAnti-IL10 antibody |
Information sourced from Uniprot.org