• Immunofluorescence analysis of L929 cells using IGFBP4 Rabbit polyclonal antibody (STJ26194) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of IGFBP4 in paraffin-embedded mouse brain using IGFBP4 Rabbit polyclonal antibody (STJ26194) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IGFBP4 in paraffin-embedded human stomach using IGFBP4 Rabbit polyclonal antibody (STJ26194) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IGFBP4 in paraffin-embedded rat brain using IGFBP4 Rabbit polyclonal antibody (STJ26194) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using IGFBP4 Rabbit polyclonal antibody (STJ26194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IGFBP4 antibody (22-258) (STJ26194)

SKU:
STJ26194

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IGFBP4 (22-258) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGFBP4
Gene ID: 3487
Uniprot ID: IBP4_HUMAN
Immunogen Region: 22-258
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-258 of human IGFBP4 (NP_001543.2).
Immunogen Sequence: DEAIHCPPCSEEKLARCRPP VGCEELVREPGCGCCATCAL GLGMPCGVYTPRCGSGLRCY PPRGVEKPLHTLMHGQGVCM ELAEIEAIQESLQPSDKDEG DHPNNSFSPCSAHDRRCLQK HFAKIRDRSTSGGKMKVNGA PREDARPVPQGSCQSELHRA LERLAASQSRTHEDLYIIPI PNCDRNGNFHPKQCHPALDG QRGKCWCVDRKTGVKLPGGL EPKGELDCHQLADSFRE
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Protein Name Insulin-Like Growth Factor-Binding Protein 4
Ibp-4
Igf-Binding Protein 4
Igfbp-4
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor-Binding Protein 4 antibody
Anti-Ibp-4 antibody
Anti-Igf-Binding Protein 4 antibody
Anti-Igfbp-4 antibody
Anti-IGFBP4 antibody
Anti-IBP4 antibody

Information sourced from Uniprot.org

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