• Immunofluorescence analysis of U2OS cells using IGF2BP2/IMP2 antibody (STJ24141) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using IGF2BP2/IMP2 antibody (STJ24141) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using IGF2BP2/IMP2 antibody (STJ24141) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IGF2BP2 antibody (500-599) (STJ24141)

SKU:
STJ24141

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IGF2BP2 (500-599) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGF2BP2
Gene ID: 10644
Uniprot ID: IF2B2_HUMAN
Immunogen Region: 500-599
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-599 of human IGF2BP2/IMP2 (NP_006539.3).
Immunogen Sequence: NFFNPKEEVKLEAHIRVPSS TAGRVIGKGGKTVNELQNLT SAEVIVPRDQTPDENEEVIV RIIGHFFASQTAQRKIREIV QQVKQQEQKYPQGVASQRSK
Tissue Specificity Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
Function RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD.
Protein Name Insulin-Like Growth Factor 2 Mrna-Binding Protein 2
Igf2 Mrna-Binding Protein 2
Imp-2
Hepatocellular Carcinoma Autoantigen P62
Igf-Ii Mrna-Binding Protein 2
Vickz Family Member 2
Database Links Reactome: R-HSA-428359
Cellular Localisation Nucleus
Cytoplasm
P-Body
Stress Granule
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Localizes At The Connecting Piece And The Tail Of The Spermatozoa
In Response To Cellular Stress
Such As Oxidative Stress
Recruited To Stress Granules
Alternative Antibody Names Anti-Insulin-Like Growth Factor 2 Mrna-Binding Protein 2 antibody
Anti-Igf2 Mrna-Binding Protein 2 antibody
Anti-Imp-2 antibody
Anti-Hepatocellular Carcinoma Autoantigen P62 antibody
Anti-Igf-Ii Mrna-Binding Protein 2 antibody
Anti-Vickz Family Member 2 antibody
Anti-IGF2BP2 antibody
Anti-IMP2 antibody
Anti-VICKZ2 antibody

Information sourced from Uniprot.org

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