• Western blot analysis of various lysates using Mouse IgE Rabbit monoclonal antibody (STJ11105043) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Negative control (NC) : Mouse brainExposure time: 120s.

Anti-IgE antibody (50-388 aa) [S5043RM] (STJ11105043)

SKU:
STJ11105043

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5043RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 50-388 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 50-388 of mouse IgE. (AAZ05128.1).
Immunogen Sequence: FNESRTILVRPVNITEPTLE LLHSSCDPNAFHSTIQLYCF IYGHILNDVSVSWLMDDREI TDTLAQTVLIKEEGKLASTC SKLNITEQQWMSESTFTCKV TSQGVDYLAHTRRCPDHEPR GVITYLIPPSPLDLYQNGAP KLTCLVVDLESEKNVNVTWN QEKKTSVSASQWYTKHHNNA TTSITSILPVVAKDWIEGYG YQCIVDHPDFPKPIVRSITK TPGQRSAPEVYVFPPPEEE
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance