![Western blot analysis of lysates from wild type (WT) and IFNAR2 Rabbit polyclonal antibody knockout (KO) HeLa cells, using [KO Validated] IFNAR2 Rabbit polyclonal antibody (STJ11104355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176999/368164/STJ11104355_1__47959.1713133674.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IFNAR2 Rabbit polyclonal antibody knockout (KO) HeLa cells, using [KO Validated] IFNAR2 Rabbit polyclonal antibody (STJ11104355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of various lysates, using [KO Validated] IFNAR2 Rabbit polyclonal antibody (STJ11104355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176999/368165/STJ11104355_2__79452.1713133675.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] IFNAR2 Rabbit polyclonal antibody (STJ11104355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and IFNAR2 Rabbit polyclonal antibody knockout (KO) HeLa cells, using [KO Validated] IFNAR2 Rabbit polyclonal antibody (STJ11104355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/176999/368164/STJ11104355_1__47959.1713133674.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IFNAR2 Rabbit polyclonal antibody knockout (KO) HeLa cells, using [KO Validated] IFNAR2 Rabbit polyclonal antibody (STJ11104355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
Anti-IFNAR2 antibody (101-243) (STJ11104355)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IFNAR2 (101-243) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IFNAR2 |
| Gene ID | 3455 |
| Uniprot ID | INAR2_HUMAN |
| Immunogen Region | 101-243 |
| Immunogen Sequence | STHEAYVTVLEGFSGNTTLF SCSHNFWLAIDMSFEPPEFE IVGFTNHINVMVKFPSIVEE ELQFDLSLVIEEQSEGIVKK HKPEIKGNMSGNFTYIIDKL IPNTNYCVSVYLEHSDEQAV IKSPLKCTLLPPGQESESAE SAK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 101-243 of human IFNAR2 (NP_997468.1). |
Additional Info
| Tissue Specificity | Isoform 3 is detected in the urine (at protein level). Expressed in blood cells. Expressed in lymphoblastoid and fibrosarcoma cell lines. |
| Post Translational Modifications | Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types. Glycosylated. |
| Function | Together with IFNAR1, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors (STAT1, STAT2 and STAT). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes. Isoform 3: Potent inhibitor of type I IFN receptor activity. |
| Protein Name | Interferon Alpha/Beta Receptor 2Ifn-R-2Ifn-Alpha Binding ProteinIfn-Alpha/Beta Receptor 2Interferon Alpha Binding ProteinType I Interferon Receptor 2 |
| Database Links | Reactome: R-HSA-909733 P48551-2Reactome: R-HSA-912694 P48551-2Reactome: R-HSA-9679191 P48551-2Reactome: R-HSA-9705671 P48551-2Reactome: R-HSA-9833109 P48551-2 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Cell MembraneIsoform 3: Secreted |
| Alternative Antibody Names | Anti-Interferon Alpha/Beta Receptor 2 antibodyAnti-Ifn-R-2 antibodyAnti-Ifn-Alpha Binding Protein antibodyAnti-Ifn-Alpha/Beta Receptor 2 antibodyAnti-Interferon Alpha Binding Protein antibodyAnti-Type I Interferon Receptor 2 antibodyAnti-IFNAR2 antibodyAnti-IFNABR antibodyAnti-IFNARB antibody |
Information sourced from Uniprot.org