Anti-IER5 antibody (Internal) (STJ71089)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-IER5 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:2000.WB: Customer's experiments gave a band at approx 45kDa in lysates of transfected H1299 overexpressing IER5 but not when transfected with empty vector (calculated MW of 33.7kDa according to |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | IER5 |
| Gene ID | 51278 |
| Uniprot ID | IER5_HUMAN |
| Accession Number | NP_057629.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | QPPSGGEDDDAEE |
Additional Info
| Function | Plays a role as a transcription factor. Mediates positive transcriptional regulation of several chaperone genes during the heat shock response in a HSF1-dependent manner. Mediates negative transcriptional regulation of CDC25B expression. Plays a role in the dephosphorylation of the heat shock factor HSF1 and ribosomal protein S6 kinase (S6K) by the protein phosphatase PP2A. Involved in the regulation of cell proliferation and resistance to thermal stress. Involved in the cell cycle checkpoint and survival in response to ionizing radiation. Associates with chromatin to the CDC25B promoter. |
| Protein Name | Immediate Early Response Gene 5 Protein |
| Cellular Localisation | NucleusCytoplasmPredominantly CytoplasmicTranslocated In The Nucleus During Heat Shock |
| Alternative Antibody Names | Anti-Immediate Early Response Gene 5 Protein antibodyAnti-IER5 antibodyAnti-PP4583 antibodyAnti-SBBI48 antibody |
Information sourced from Uniprot.org