• Immunohistochemistry analysis of IER5 in paraffin-embedded Rat pancreas using IER5 Rabbit polyclonal antibody (STJ11100411) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IER5 in paraffin-embedded Human esophageal using IER5 Rabbit polyclonal antibody (STJ11100411) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using IER5 Rabbit polyclonal antibody (STJ11100411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IER5 antibody (200-300) (STJ11100411)

SKU:
STJ11100411

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IER5
Gene ID: 51278
Uniprot ID: IER5_HUMAN
Immunogen Region: 200-300
Specificity: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human IER5 (NP_057629.2).
Immunogen Sequence: APQPAEDEPPAPPAVCPRKR CAAGVGGGPAGCPAPGSTPL KKPRRNLEQPPSGGEDDDAE EMETGNVANLISIFGSSFSG LLRKSPGGGREEEEGEESGP E
Tissue Specificity Expressed in acute myeloid leukemia (AML) cells.
Function Plays a role as a transcription factor. Mediates positive transcriptional regulation of several chaperone genes during the heat shock response in a HSF1-dependent manner. Mediates negative transcriptional regulation of CDC25B expression. Plays a role in the dephosphorylation of the heat shock factor HSF1 and ribosomal protein S6 kinase (S6K) by the protein phosphatase PP2A. Involved in the regulation of cell proliferation and resistance to thermal stress. Involved in the cell cycle checkpoint and survival in response to ionizing radiation. Associates with chromatin to the CDC25B promoter.
Protein Name Immediate Early Response Gene 5 Protein
Cellular Localisation Nucleus
Cytoplasm
Predominantly Cytoplasmic
Translocated In The Nucleus During Heat Shock
Alternative Antibody Names Anti-Immediate Early Response Gene 5 Protein antibody
Anti-IER5 antibody
Anti-PP4583 antibody
Anti-SBBI48 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance