• Western blot analysis of Jurkat, using IDH2 antibody (STJ29270) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates, using IDH2 antibody (STJ29270) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using IDH2 rabbit polyclonal antibody (STJ29270) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using IDH2 rabbit polyclonal antibody (STJ29270) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 1 Mu g IDH2 antibody (STJ29270). Western blot was performed from the immunoprecipitate using IDH2 antibody (STJ29270) at a dilution of 1:1000.
  • Chromatin immunoprecipitation of extracts of 293T cell line, using IDH2 antibody (STJ29270) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-IDH2 antibody (193-452) (STJ29270)

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STJ29270

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IDH2 (193-452) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:20-1:50
IP 1:50-1:200
ChIP 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDH2
Gene ID: 3418
Uniprot ID: IDHP_HUMAN
Immunogen Region: 193-452
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2).
Immunogen Sequence: KMVFTPKDGSGVKEWEVYNF PAGGVGMGMYNTDESISGFA HSCFQYAIQKKWPLYMSTKN TILKAYDGRFKDIFQEIFDK HYKTDFDKNKIWYEHRLIDD MVAQVLKSSGGFVWACKNYD GDVQSDILAQGFGSLGLMTS VLVCPDGKTIEAEAAHGTVT RHYREHQKGRPTSTNPIASI FAWTRGLEHRGKLDGNQDLI RFAQMLEKVCVETVESGAMT KDLAGCIHGLSNVKLNEHF
Post Translational Modifications Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
Function Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Protein Name Isocitrate Dehydrogenase Nadp - Mitochondrial
Idh
Icd-M
Idp
Nadp(+-Specific Icdh
Oxalosuccinate Decarboxylase
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Isocitrate Dehydrogenase Nadp - Mitochondrial antibody
Anti-Idh antibody
Anti-Icd-M antibody
Anti-Idp antibody
Anti-Nadp(+-Specific Icdh antibody
Anti-Oxalosuccinate Decarboxylase antibody
Anti-IDH2 antibody

Information sourced from Uniprot.org

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