Western blot analysis of lysates from mouse kidney, using ID4 Rabbit polyclonal antibody (STJ118668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ID4 antibody (1-100) (STJ118668)

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STJ118668
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ID4
Gene ID: 3400
Uniprot ID: ID4_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ID4 (NP_001537.1).
Immunogen Sequence: MKAVSPVRPSGRKAPSGCGG GELALRCLAEHGHSLGGSAA AAAAAAAARCKAAEAAADEP ALCLQCDMNDCYSRLRRLVP TIPPNKKVSKVEILQHVIDY
Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation.
Protein Name Dna-Binding Protein Inhibitor Id-4
Class B Basic Helix-Loop-Helix Protein 27
Bhlhb27
Inhibitor Of Dna Binding 4
Inhibitor Of Differentiation 4
Database Links Reactome: R-HSA-9031628
Reactome: R-HSA-9830364
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna-Binding Protein Inhibitor Id-4 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 27 antibody
Anti-Bhlhb27 antibody
Anti-Inhibitor Of Dna Binding 4 antibody
Anti-Inhibitor Of Differentiation 4 antibody
Anti-ID4 antibody
Anti-BHLHB27 antibody

Information sourced from Uniprot.org

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