![Anti-Human CD33 nanobody [SAA2052] (STJN000567)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/201180/413278/STJN000567-SDS-PAGE-1__05794.1749232759.jpg?c=1 "Anti-Human CD33 nanobody [SAA2052] (STJN000567)")
![Anti-Human CD33 nanobody [SAA2052] (STJN000567)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/201180/413278/STJN000567-SDS-PAGE-1__05794.1749232759.jpg?c=1 "Anti-Human CD33 nanobody [SAA2052] (STJN000567)")
Anti-Human CD33 nanobody [SAA2052] (STJN000567)
SPECIFICATIONS
ClonalityMonoclonal
HostAlpaca
ConjugationUnconjugated
IsotypeVHH-8His-Cys-tag
General Information
| Short Description | Alpaca monoclonal nanobody anti-Myeloid Cell Surface antigen Cd33 is suitable for use in ELISA and Flow Cytometry research applications. |
| Applications | ELISA/FC |
| Host | Alpaca |
| Reactivity | Human/Cynomolgus |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA2052 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | CD33 |
| Gene ID | 945 |
| Uniprot ID | CD33_HUMAN |
Additional Info
| Post Translational Modifications | Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent. |
| Function | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| Protein Name | Myeloid Cell Surface Antigen Cd33Sialic Acid-Binding Ig-Like Lectin 3Siglec-3Gp67Cd Antigen Cd33 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Cd33m: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Cd33m: PeroxisomeCd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes |
| Alternative Nanobody Names | Anti-Myeloid Cell Surface Antigen Cd33 nanobodyAnti-Sialic Acid-Binding Ig-Like Lectin 3 nanobodyAnti-Siglec-3 nanobodyAnti-Gp67 nanobodyAnti-Cd Antigen Cd33 nanobodyAnti-CD33 nanobodyAnti-SIGLEC3 nanobody |
Information sourced from Uniprot.org