Anti-HTRA2 antibody [8G11-4B8-4F10-G5] (STJ99217)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenRecombinant human HtrA2/Omi protein.
General Information
| Short Description | Mouse monoclonal anti-Serine protease HTRA2, mitochondrial for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 8G11-4B8-4F10-G5 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HTRA2 |
| Gene ID | 27429 |
| Uniprot ID | HTRA2_HUMAN |
| Immunogen | Recombinant human HtrA2/Omi protein. |
| Specificity | This antibody detects endogenous levels of HtrA2/Omi and does not cross-react with related proteins. |
Additional Info
| Post Translational Modifications | Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC. Autoproteolytically activated. |
| Function | Isoform 1: Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity.or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2: Seems to be proteolytically inactive. |
| Protein Name | Serine Protease Htra2 - MitochondrialHigh Temperature Requirement Protein A2Htra2Omi Stress-Regulated EndoproteaseSerine Protease 25Serine Proteinase Omi |
| Database Links | Reactome: R-HSA-9837999Reactome: R-HSA-9841251 |
| Cellular Localisation | Mitochondrion Intermembrane SpaceMitochondrion MembraneSingle-Pass Membrane ProteinPredominantly Present In The Intermembrane SpaceReleased Into The Cytosol Following Apoptotic StimuliSuch As Uv TreatmentAnd Stimulation Of Mitochondria With Caspase-8 Truncated Bid/TbidIsoform 1: Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Serine Protease Htra2 - Mitochondrial antibodyAnti-High Temperature Requirement Protein A2 antibodyAnti-Htra2 antibodyAnti-Omi Stress-Regulated Endoprotease antibodyAnti-Serine Protease 25 antibodyAnti-Serine Proteinase Omi antibodyAnti-HTRA2 antibodyAnti-OMI antibodyAnti-PRSS25 antibody |
Information sourced from Uniprot.org