• Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g HTRA2 antibody (STJ117077). Western blot was performed from the immunoprecipitate using HTRA2 antibody (STJ117077) at a dilution of 1:1000.
  • Western blot analysis of lysates from MCF7 cells, using HTRA2 Rabbit polyclonal antibody (STJ117077) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HTRA2 antibody (334-458) (STJ117077)

SKU:
STJ117077

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HTRA2 (334-458) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTRA2
Gene ID: 27429
Uniprot ID: HTRA2_HUMAN
Immunogen Region: 334-458
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 334-458 of human HTRA2 (NP_037379.1).
Immunogen Sequence: PSDRLREFLHRGEKKNSSSG ISGSQRRYIGVMMLTLSPSI LAELQLREPSFPDVQHGVLI HKVILGSPAHRAGLRPGDVI LAIGEQMVQNAEDVYEAVRT QSQLAVQIRRGRETLTLYVT PEVTE
Tissue Specificity Isoform 1: Ubiquitously expressed.
Post Translational Modifications Autoproteolytically activated.
Function Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
Protein Name Serine Protease Htra2 - Mitochondrial
High Temperature Requirement Protein A2
Htra2
Omi Stress-Regulated Endoprotease
Serine Protease 25
Serine Proteinase Omi
Cellular Localisation Mitochondrion Intermembrane Space
Mitochondrion Membrane
Single-Pass Membrane Protein
Predominantly Present In The Intermembrane Space
Released Into The Cytosol Following Apoptotic Stimuli
Such As Uv Treatment
And Stimulation Of Mitochondria With Caspase-8 Truncated Bid/Tbid
Isoform 1: Endoplasmic Reticulum
Alternative Antibody Names Anti-Serine Protease Htra2 - Mitochondrial antibody
Anti-High Temperature Requirement Protein A2 antibody
Anti-Htra2 antibody
Anti-Omi Stress-Regulated Endoprotease antibody
Anti-Serine Protease 25 antibody
Anti-Serine Proteinase Omi antibody
Anti-HTRA2 antibody
Anti-OMI antibody
Anti-PRSS25 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance