Anti-HSPE1 antibody (50-150) [S0MR] (STJ11102310)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Cpn10 (50-150) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S0MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HSPE1 |
| Gene ID | 3336 |
| Uniprot ID | CH10_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | GSGSKGKGGEIQPVSVKVGD KVLLPEYGGTKVVLDDKDYF LFRDGDILGKYVD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 50-150 of human HSPE1/HSP10/CPN10 (P61604). |
Additional Info
| Function | Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable). |
| Protein Name | 10 Kda Heat Shock Protein - MitochondrialHsp1010 Kda ChaperoninChaperonin 10Cpn10Early-Pregnancy FactorEpfHeat Shock Protein Family E Member 1 |
| Database Links | Reactome: R-HSA-9013408Reactome: R-HSA-9841251 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-10 Kda Heat Shock Protein - Mitochondrial antibodyAnti-Hsp10 antibodyAnti-10 Kda Chaperonin antibodyAnti-Chaperonin 10 antibodyAnti-Cpn10 antibodyAnti-Early-Pregnancy Factor antibodyAnti-Epf antibodyAnti-Heat Shock Protein Family E Member 1 antibodyAnti-HSPE1 antibody |
Information sourced from Uniprot.org