• Immunofluorescence analysis of U2OS cells using Grp75/MOT/Grp75/MOT/HSPA9 antibody (STJ24100). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using Grp75/MOT/Grp75/MOT/HSPA9 antibody (STJ24100) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Grp75/MOT/Grp75/MOT/HSPA9 antibody (STJ24100) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using Grp75/MOT/Grp75/MOT/HSPA9 antibody (STJ24100) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Grp75/MOT/Grp75/MOT/HSPA9 antibody (STJ24100) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Grp75/MOT/Grp75/MOT/HSPA9 antibody (STJ24100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-HSPA9 antibody (380-679) (STJ24100)

SKU:
STJ24100

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSPA9 (380-679) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPA9
Gene ID: 3313
Uniprot ID: GRP75_HUMAN
Immunogen Region: 380-679
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-679 of human Grp75/MOT/Grp75/MOT/HSPA9 (NP_004125.3).
Immunogen Sequence: IGEVILVGGMTRMPKVQQTV QDLFGRAPSKAVNPDEAVAI GAAIQGGVLAGDVTDVLLLD VTPLSLGIETLGGVFTKLIN RNTTIPTKKSQVFSTAADGQ TQVEIKVCQGEREMAGDNKL LGQFTLIGIPPAPRGVPQIE VTFDIDANGIVHVSAKDKGT GREQQIVIQSSGGLSKDDIE NMVKNAEKYAEEDRRKKERV EAVNMAEGIIHDTETKMEEF KDQLPADECNKLKEEISKM
Function Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU. Regulates erythropoiesis via stabilization of ISC assembly. May play a role in the control of cell proliferation and cellular aging.
Protein Name Stress-70 Protein - Mitochondrial
75 Kda Glucose-Regulated Protein
Grp-75
Heat Shock 70 Kda Protein 9
Mortalin
Mot
Peptide-Binding Protein 74
Pbp74
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-3371453
Reactome: R-HSA-8949613
Reactome: R-HSA-8950505
Cellular Localisation Mitochondrion
Nucleus
Nucleolus
Alternative Antibody Names Anti-Stress-70 Protein - Mitochondrial antibody
Anti-75 Kda Glucose-Regulated Protein antibody
Anti-Grp-75 antibody
Anti-Heat Shock 70 Kda Protein 9 antibody
Anti-Mortalin antibody
Anti-Mot antibody
Anti-Peptide-Binding Protein 74 antibody
Anti-Pbp74 antibody
Anti-HSPA9 antibody
Anti-GRP75 antibody
Anti-HSPA9B antibody
Anti-mt-HSP70 antibody

Information sourced from Uniprot.org

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