Anti-HSP70 antibody [3G10] (STJ96944)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenSynthetic Peptide of HSP70
General Information
| Short Description | Mouse monoclonal anti-Heat shock 70 kDa protein 1-like and OBSOLETE for use in WB, IHC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 3G10 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:1000-2000IF 1:100-200IHC 1:50-300 |
| Formulation | Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HSPA1L |
| Gene ID | 3305 |
| Uniprot ID | HS71L_HUMAN |
| Immunogen | Synthetic Peptide of HSP70 |
| Specificity | The antibody detects endogenous HSP70 proteins. |
Additional Info
| Function | Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Positive regulator of PRKN translocation to damaged mitochondria. |
| Protein Name | Heat Shock 70 Kda Protein 1-LikeHeat Shock 70 Kda Protein 1lHeat Shock 70 Kda Protein 1-HomHsp70-HomHeat Shock Protein Family A Member 1l |
| Database Links | Reactome: R-HSA-3371453Reactome: R-HSA-3371497Reactome: R-HSA-3371568Reactome: R-HSA-3371571Reactome: R-HSA-9833482 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Heat Shock 70 Kda Protein 1-Like antibodyAnti-Heat Shock 70 Kda Protein 1l antibodyAnti-Heat Shock 70 Kda Protein 1-Hom antibodyAnti-Hsp70-Hom antibodyAnti-Heat Shock Protein Family A Member 1l antibodyAnti-HSPA1L antibody |
Information sourced from Uniprot.org