• Western blot analysis of Hsp60. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:3000, RT, 1h.
  • Immunohistochemistry analysis of Hsp60. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of Hsp60. Sample: Human colon cancer, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of Hsp60. Sample: mouse kidney, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of Hsp60. Sample: Rat kidney, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.
  • ICC analysis of Hsp60green. Sample: HeLa, 4% PFA 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:100, 4C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG, 1:400, RT, 1h.
  • ICC analysis of Hsp60green. Sample: NIH3T3, 4% PFA 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:100, 4C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG, 1:400, RT, 1h.

Anti-Hsp60 antibody [SM5082] (STJA0005082)

SKU:
STJA0005082

£170.50 - £233.50
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Host: Mouse
Applications: WB/IHC/IF/ICC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Hsp60 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: SM5082
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (H, M, R) 1:500-1:1000
IHC/IF (H, M, R) 1:500-1:1000
ICC/IF (H, M) 1:100-1:400
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Hspd1
Gene ID: 15510
Uniprot ID: CH60_MOUSE
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse HSP60
Protein Name 60 Kda Heat Shock Protein - Mitochondrial
60 Kda Chaperonin
Chaperonin 60
Cpn60
Hsp-65
Heat Shock Protein 60
Hsp-60
Hsp60
Mitochondrial Matrix Protein P1
Database Links Reactome: R-MMU-1268020
Reactome: -MMU-9837999
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-60 Kda Heat Shock Protein - Mitochondrial antibody
Anti-60 Kda Chaperonin antibody
Anti-Chaperonin 60 antibody
Anti-Cpn60 antibody
Anti-Hsp-65 antibody
Anti-Heat Shock Protein 60 antibody
Anti-Hsp-60 antibody
Anti-Hsp60 antibody
Anti-Mitochondrial Matrix Protein P1 antibody
Anti-Hspd1 antibody
Anti-Hsp60 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance