| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1-steroid dehydrogenase (30-110 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HSD3B1 |
| Gene ID: | 3283 |
| Uniprot ID: | 3BHS1_HUMAN |
| Immunogen Region: | 30-110 aa |
| Specificity: | 3BHS1 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 30-110 |
| Function | A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable). |
| Protein Name | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I 3-Beta-Hsd I 3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase 3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase 3-Beta-Hydroxysteroid 3-Dehydrogenase Delta-5-3-Ketosteroid Isomerase Dihydrotestosterone Oxidoreductase Steroid Delta-Isomerase Trophoblast Antigen Fdo161g |
| Database Links | Reactome: R-HSA-193048 Reactome: R-HSA-193993 Reactome: R-HSA-194002 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Membrane Protein Mitochondrion Membrane |
| Alternative Antibody Names | Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 antibody Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I antibody Anti-3-Beta-Hsd I antibody Anti-3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase antibody Anti-3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase antibody Anti-3-Beta-Hydroxysteroid 3-Dehydrogenase antibody Anti-Delta-5-3-Ketosteroid Isomerase antibody Anti-Dihydrotestosterone Oxidoreductase antibody Anti-Steroid Delta-Isomerase antibody Anti-Trophoblast Antigen Fdo161g antibody Anti-HSD3B1 antibody Anti-3BH antibody Anti-HSDB3A antibody |
Information sourced from Uniprot.org
