• Western blot analysis of lysate from K562 cells, using HPSE Antibody.
  • Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100
  • Western blot analysis of K562 cells using HPA1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HPSE antibody (241-290 aa) (STJ96718)

SKU:
STJ96718

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Heparanase (241-290 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HPSE
Gene ID: 10855
Uniprot ID: HPSE_HUMAN
Immunogen Region: 241-290 aa
Specificity: HPA1 Polyclonal Antibody detects endogenous levels of HPA1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human HPSE at the amino acid range 241-290
Post Translational Modifications Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme. N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
Function Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
Protein Name Heparanase
Endo-Glucoronidase
Heparanase-1
Hpa1 Cleaved Into - Heparanase 8 Kda Subunit - Heparanase 50 Kda Subunit
Database Links Reactome: R-HSA-2024096
Reactome: R-HSA-6798695
Cellular Localisation Lysosome Membrane
Peripheral Membrane Protein
Secreted
Nucleus
Proheparanase Is Secreted Via Vesicles Of The Golgi
Interacts With Cell Membrane Heparan Sulfate Proteoglycans (Hspgs)
Endocytosed And Accumulates In Endosomes
Transferred To Lysosomes Where It Is Proteolytically Cleaved To Produce The Active Enzyme
Under Certain Stimuli
Transferred To The Cell Surface
Associates With Lipid Rafts
Colocalizes With Sdc1 In Endosomal/Lysosomal Vesicles
Accumulates In Perinuclear Lysosomal Vesicles
Heparin Retains Proheparanase In The Extracellular Medium
Alternative Antibody Names Anti-Heparanase antibody
Anti-Endo-Glucoronidase antibody
Anti-Heparanase-1 antibody
Anti-Hpa1 Cleaved Into - Heparanase 8 Kda Subunit - Heparanase 50 Kda Subunit antibody
Anti-HPSE antibody
Anti-HEP antibody
Anti-HPA antibody
Anti-HPA1 antibody
Anti-HPR1 antibody
Anti-HPSE1 antibody
Anti-HSE1 antibody

Information sourced from Uniprot.org

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