• Immunohistochemistry analysis of paraffin-embedded mouse kidney using HPD antibody (STJ28588) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using HPD antibody (STJ28588) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HPD antibody (STJ28588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-HPD antibody (1-393) (STJ28588)

SKU:
STJ28588

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HPD
Gene ID: 3242
Uniprot ID: HPPD_HUMAN
Immunogen Region: 1-393
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human HPD (NP_002141.1).
Immunogen Sequence: MTTYSDKGAKPERGRFLHFH SVTFWVGNAKQAASFYCSKM GFEPLAYRGLETGSREVVSH VIKQGKIVFVLSSALNPWNK EMGDHLVKHGDGVKDIAFEV EDCDYIVQKARERGAKIMRE PWVEQDKFGKVKFAVLQTYG DTTHTLVEKMNYIGQFLPGY EAPAFMDPLLPKLPKCSLEM IDHIVGNQPDQEMVSASEWY LKNLQFHRFWSVDDTQVHTE YSSLRSIVVANYEESIKMP
Function Catalyzes the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, one of the steps in tyrosine catabolism.
Protein Name 4-Hydroxyphenylpyruvate Dioxygenase
4-Hydroxyphenylpyruvic Acid Oxidase
4hppd
Hpd
Hppdase
Database Links Reactome: R-HSA-8963684
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Golgi Apparatus Membrane
Alternative Antibody Names Anti-4-Hydroxyphenylpyruvate Dioxygenase antibody
Anti-4-Hydroxyphenylpyruvic Acid Oxidase antibody
Anti-4hppd antibody
Anti-Hpd antibody
Anti-Hppdase antibody
Anti-HPD antibody
Anti-PPD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance