| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/FC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-HP alpha chain (Internal) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 18kDa band observed in Human Liver lysates and approx. 20kDa in preliminary testing of HepG2 cell lysate (calculated MW of 18kDa according to alpha chain cleaved from NP_005134. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
HP |
| Gene ID: |
3240 |
| Uniprot ID: |
HPT_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_005134.1; NP_001119574.1 |
| Specificity: |
This antibody is expected to recognize both reported isoforms (NP_005134.1; NP_001119574.1). |
| Immunogen Sequence: |
QCKNYYKLRTEGD |
| Function | As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. |
| Protein Name | HaptoglobinZonulin Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain |
| Database Links | Reactome: R-HSA-2168880Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Haptoglobin antibodyAnti-Zonulin Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain antibodyAnti-HP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
