| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HMGA1 |
| Gene ID: | 3159 |
| Uniprot ID: | HMGA1_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1). |
| Immunogen Sequence: | MSESSSKSSQPLASKQEKDG TEKRGRGRPRKQPPVSPGTA LVGSQKEPSEVPTPKRPRGR PKGSKNKGAAKTRKTTTTPG RKPRGRPKKLEKEEEEGISQ |
| Post Translational Modifications | Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity. HMG-Y is not methylated. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60. |
| Function | HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. |
| Protein Name | High Mobility Group Protein Hmg-I/Hmg-Y Hmg-I(Y High Mobility Group At-Hook Protein 1 High Mobility Group Protein A1 High Mobility Group Protein R |
| Database Links | Reactome: R-HSA-162592 Reactome: R-HSA-164843 Reactome: R-HSA-175567 Reactome: R-HSA-177539 Reactome: R-HSA-180689 Reactome: R-HSA-180910 Reactome: R-HSA-2559584 |
| Cellular Localisation | Nucleus Chromosome |
| Alternative Antibody Names | Anti-High Mobility Group Protein Hmg-I/Hmg-Y antibody Anti-Hmg-I(Y antibody Anti-High Mobility Group At-Hook Protein 1 antibody Anti-High Mobility Group Protein A1 antibody Anti-High Mobility Group Protein R antibody Anti-HMGA1 antibody Anti-HMGIY antibody |
Information sourced from Uniprot.org