• Western blot analysis of extracts of various cell lines, using HMBS Rabbit monoclonal antibody (STJ11101395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using HMBS Rabbit monoclonal antibody (STJ11101395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-HMBS antibody (250-350) [S5MR] (STJ11101395)

SKU:
STJ11101395

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HMBS (250-350) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMBS
Gene ID: 3145
Uniprot ID: HEM3_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HMBS (P08397).
Immunogen Sequence: ERAFLRHLEGGCSVPVAVHT AMKDGQLYLTGGVWSLDGSD SIQETMQATIHVPAQHEDGP EDDPQLVGITARNIPRGPQL AAQNLGISLANLLLSKGAKN I
Tissue Specificity Isoform 1: Is ubiquitously expressed. Isoform 2: Is found only in erythroid cells.
Function As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact.
Protein Name Porphobilinogen Deaminase
Pbg-D
Hydroxymethylbilane Synthase
Hmbs
Pre-Uroporphyrinogen Synthase
Database Links Reactome: R-HSA-189451
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Porphobilinogen Deaminase antibody
Anti-Pbg-D antibody
Anti-Hydroxymethylbilane Synthase antibody
Anti-Hmbs antibody
Anti-Pre-Uroporphyrinogen Synthase antibody
Anti-HMBS antibody
Anti-PBGD antibody
Anti-UPS antibody

Information sourced from Uniprot.org

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