• Immunofluorescence analysis of U2OS cells using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Hexokinase II in paraffin-embedded rat heart using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Hexokinase II in paraffin-embedded mouse heart using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Hexokinase II in paraffin-embedded human colon using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Hexokinase II in paraffin-embedded human breast cancer using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using Hexokinase II Rabbit polyclonal antibody (STJ24020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HK2 antibody (1-120) (STJ24020)

SKU:
STJ24020

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HK2 (1-120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HK2
Gene ID: 3099
Uniprot ID: HXK2_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).
Immunogen Sequence: MIASHLLAYFFTELNHDQVQ KVDQYLYHMRLSDETLLEIS KRFRKEMEKGLGATTHPTAA VKMLPTFVRSTPDGTEHGEF LALDLGGTNFRVLWVKVTDN GLQKVEMENQIYAIPEDIMR
Tissue Specificity Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
Function Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis.
Protein Name Hexokinase-2
Hexokinase Type Ii
Hk Ii
Hexokinase-B
Muscle Form Hexokinase
Database Links Reactome: R-HSA-70171
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
The Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane
The Interaction With The Mitochondrial Outer Membrane Via The Mitochondrial-Binding Peptide (Mbp) Region Promotes Higher Stability Of The Protein
Release From The Mitochondrial Outer Membrane Into The Cytosol Induces Permeability Transition Pore (Ptp) Opening And Apoptosis
Alternative Antibody Names Anti-Hexokinase-2 antibody
Anti-Hexokinase Type Ii antibody
Anti-Hk Ii antibody
Anti-Hexokinase-B antibody
Anti-Muscle Form Hexokinase antibody
Anti-HK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance