Host: |
Mouse |
Applications: |
ELISA/WB/ICC |
Reactivity: |
HIV-1 |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Mouse monoclonal antibody anti-HIV-1 Nef (35-50 aa) is suitable for use in ELISA, Western Blot and Immunocytochemistry research applications. |
Clonality: |
Monoclonal |
Clone ID: |
3D12 |
Conjugation: |
Unconjugated |
Isotype: |
IgG1 |
Formulation: |
PBS pH 7.4, with 0.1% sodium azide |
Purification: |
Protein G purification NA |
Dilution Range: |
ELISA 0.1 to 0.2 µg/ml; WB 2 µg/ml. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
Storage Instruction: |
Store at-20 to-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
Immunogen Region: |
35-50 aa |
Specificity: |
Reacts with HIV-1 subtypes B and C, BRU, IIIB, and HAN strains. Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA-HXB2) |
Immunogen: |
Recombinant Nef protein of HIV-1 HXB2 (purified from E. coli). Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA-HXB2) |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance