| Host: | Mouse |
| Applications: | ELISA/WB |
| Reactivity: | HIV-1B/HIV-1C |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-HIV-1 Nef (150-158 aa) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 2F2 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS pH 7.4, with 0.1% sodium azide |
| Purification: | NA Protein G purification NA |
| Dilution Range: | ELISA 0.05 to 0.1 µg/ml; WB 2 µg/ml Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Storage Instruction: | Store at-20 to-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Immunogen Region: | 150-158 aa |
| Specificity: | Reacts with HIV-1 Nef subtypes B and C; BRU, and IIIB strains. Mapped to amino acids 150-158 (PDKVEEANK, HIV-1 BRU; PEKVEEANE – B subtype consensus) |
| Immunogen: | Recombinant Nef protein of HIV-1 BRU (purified from E. coli). Mapped to amino acids 150-158 (PDKVEEANK, HIV-1 BRU; PEKVEEANE – B subtype consensus) |