• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H3 antibody (STJ116178) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 150 Mu g extracts of MCF7 cells using 3 Mu g Histone H3 antibody (STJ116178). Western blot was performed from the immunoprecipitate using Histone H3 antibody (STJ116178) at a dilution of 1:500.
  • Immunohistochemistry analysis of Histone H3 in paraffin-embedded rat ovary using Histone H3 Rabbit polyclonal antibody (STJ116178) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H3 in paraffin-embedded mouse liver using Histone H3 Rabbit polyclonal antibody (STJ116178) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H3 in paraffin-embedded human breast cancer using Histone H3 Rabbit polyclonal antibody (STJ116178) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Histone H3 Rabbit polyclonal antibody (STJ116178) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Histone H3 antibody (50-136) (STJ116178)

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STJ116178

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone H3 (50-136) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IHC-P 1:50-1:200
IP 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 50-136
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-136 of human HIST3H3 (NP_003484.1).
Immunogen Sequence: REIRRYQKSTELLIRKLPFQ RLMREIAQDFKTDLRFQSSA VMALQEACESYLVGLFEDTN LCVIHAKRVTIMPKDIQLAR RIRGERA
Background This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1, 4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

Information sourced from Uniprot.org

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