Anti-Histone H1 antibody (1-50 aa) (STJ93515)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Histone H1 at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-Histone H1.5 and Histone H1.3 and Histone H1.4 (1-50 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | H1-5H1-4300930083007H15_HUMANH14_HUMANH13_HUMAN< |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Histone H1 at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | Histone H1 Polyclonal Antibody detects endogenous levels of Histone H1 protein. |
Additional Info
| Post Translational Modifications | H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Phosphorylated at Thr-11 by GSK3B during mitosis in prometaphase and dephosphorylated in telophase. Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. |
| Function | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. |
| Protein Name | Histone H1.5Histone H1aHistone H1bHistone H1s-3 |
| Database Links | Reactome: R-HSA-140342Reactome: R-HSA-2559584 |
| Cellular Localisation | NucleusChromosomeMore Commonly Found In HeterochromatinAssociates With Actively Transcribed Chromatin And Not Heterochromatin |
| Alternative Antibody Names | Anti-Histone H1.5 antibodyAnti-Histone H1a antibodyAnti-Histone H1b antibodyAnti-Histone H1s-3 antibodyAnti-H1-5 antibodyAnti-H1F5 antibodyAnti-HIST1H1B antibody |
Information sourced from Uniprot.org