| Host: |
Rabbit |
| Applications: |
WB/IHC-F/IHC-P/ICC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Histone H1.3 (5-17 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
R09-8H7 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: |
Affinity Purified |
| Concentration: |
0.3 mg/ml |
| Dilution Range: |
WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Storage Instruction: |
Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: |
H1-3 |
| Gene ID: |
3007 |
| Uniprot ID: |
H13_HUMAN |
| Immunogen Region: |
5-17 aa |
| Immunogen: |
A synthetic peptide of human Histone H1.3 |
| Post Translational Modifications | H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Citrullination at Arg-55 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. |
| Function | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. |
| Protein Name | Histone H1.3Histone H1cHistone H1s-2 |
| Database Links | Reactome: R-HSA-140342Reactome: R-HSA-2559584 |
| Cellular Localisation | NucleusChromosomeMore Commonly Found In EuchromatinIs Associated With Inactive Chromatin |
| Alternative Antibody Names | Anti-Histone H1.3 antibodyAnti-Histone H1c antibodyAnti-Histone H1s-2 antibodyAnti-H1-3 antibodyAnti-H1F3 antibodyAnti-HIST1H1D antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
