| Short Description : | Rabbit monoclonal anti-His-Tag for use in WB, IHC-P, ICC, IF, FC and IP in Species-independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ICC/IF/FC/IP |
| Host: | Rabbit |
| Reactivity: | Species-independent |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Purification: | Affinity Purified |
| Dilution Range: | WB-1:1000-1:5000IHC-P-1:1000-1:5000ICC/IF-1:100FC-1:200-1:500IP-1:20-1:50 |
| Formulation: | Liquid in 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Immunogen: | Synthetic peptide: HHHHHH conjugated to KLH. |
| Background | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N-or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. |
Information sourced from Uniprot.org
