Anti-His-Tag antibody (STJA0035427)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide: HHHHHH conjugated to KLH.
General Information
| Short Description | Rabbit monoclonal anti-His-Tag for use in WB, IHC-P, ICC, IF, FC and IP in Species-independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/FC/IP |
| Host | Rabbit |
| Reactivity | Species-independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB-1:1000-1:5000IHC-P-1:1000-1:5000ICC/IF-1:100FC-1:200-1:500IP-1:20-1:50 |
| Formulation | Liquid in 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Immunogen | Synthetic peptide: HHHHHH conjugated to KLH. |
Additional Info
| Background | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N-or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. |
Information sourced from Uniprot.org