• Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using rabbit anti His-Tag polyclonal antibody antibody (STJ11103707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells using rabbit anti His-Tag antibody (STJ11103707) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-Tag with 3 Mu g rabbit anti His-Tag polyclonal antibody antibody (STJ11103707). Western blot was performed from the immunoprecipitate using rabbit anti His-Tag polyclonal antibody antibody (STJ11103707) at 1:5000 dilution.
  • Immunoprecipitation analysis of extracts of cells using His-Tag polyclonal antibody (STJ11103707). Western blot was performed from the immunoprecipitate using His-Tag polyclonal antibody (STJ11103707) at a dilition of 1:3000.

Anti-His-Tag antibody (STJ11103707)

SKU:
STJ11103707

£72.50 - £300.50
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Host: Rabbit
Applications: WB/IF/ICC/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to His tag.
Immunogen Sequence: HHHHHH
Background Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Information sourced from Uniprot.org

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