• Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using rabbit anti His-Tag polyclonal antibody antibody (STJ11103707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells using rabbit anti His-Tag antibody (STJ11103707) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-Tag with 3 Mu g rabbit anti His-Tag polyclonal antibody antibody (STJ11103707). Western blot was performed from the immunoprecipitate using rabbit anti His-Tag polyclonal antibody antibody (STJ11103707) at 1:5000 dilution.
  • Immunoprecipitation analysis of extracts of cells using His-Tag polyclonal antibody (STJ11103707). Western blot was performed from the immunoprecipitate using His-Tag polyclonal antibody (STJ11103707) at a dilition of 1:3000.

Anti-His-Tag antibody (STJ11103707)

SKU:
STJ11103707

£72.50 - £298.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-His-Tag is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to His tag.
Immunogen Sequence: HHHHHH
Background This gene encodes a nucleoid protein localized to the mitochondria inner membrane. The encoded protein affects regulation of insulin secretion, mitochondrial ATP production, and myogenesis through modulation of mitochondrial respiratory chain activity.

Information sourced from Uniprot.org

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