| Host: | Mouse |
| Applications: | WB/ICC/IF/IP |
| Reactivity: | Species-independent |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-His-Tag for use in WB, ICC, IF and IP in Species-independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 2A2-4H2-10D8 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Purification: | Affinity Purified |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-1:10000IF 1:100-1:500IP 1:200 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Immunogen: | Synthetic peptide: HHHHHH conjugated to KLH. |
| Background | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N-or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. |
Information sourced from Uniprot.org
