• Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (STJ29094).
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using HIRIP3 antibody (STJ29094) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (STJ29094) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using HIRIP3 antibody (STJ29094) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (STJ29094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HIRIP3 antibody (417-556) (STJ29094)

SKU:
STJ29094

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HIRIP3 (417-556) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HIRIP3
Gene ID: 8479
Uniprot ID: HIRP3_HUMAN
Immunogen Region: 417-556
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2).
Immunogen Sequence: GRRGEDHPAVMRLKRYIRAC GAHRNYKKLLGSCCSHKERL SILRAELEALGMKGTPSLGK CRALKEQREEAAEVASLDVA NIISGSGRPRRRTAWNPLGE AAPPGELYRRTLDSDEERPR PAPPDWSHMRGIISSDGESN
Tissue Specificity Widely expressed. Isoform 1 is predominant in skeletal muscle. Isoform 2 is predominant in liver and heart.
Post Translational Modifications Phosphorylated by CK2.
Function May play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.
Protein Name Hira-Interacting Protein 3
Cellular Localisation Nucleus
Nuclear Throughout The Cell Cycle And Is Excluded From Condensed Chromatin During Mitosis
Alternative Antibody Names Anti-Hira-Interacting Protein 3 antibody
Anti-HIRIP3 antibody

Information sourced from Uniprot.org

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