Anti-HIRA antibody (550-675) [S8MR] (STJ11103638)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal HIRA (550-675) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S8MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HIRA |
| Gene ID | 7290 |
| Uniprot ID | HIRA_HUMAN |
| Immunogen Region | 550-675 |
| Immunogen Sequence | PSVLTTPSKIEPMKAFDSRF TERSKATPGAPALTSMTPTA VERLKEQNLVKELRPRDLLE SSSDSDEKVPLAKASSLSKR KLELEVETVEKKKKGRPRKD SRLMPVSLSVQSPAALTAEK EAMCLS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 550-675 of human HIRA (P54198). |
Additional Info
| Tissue Specificity | Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta. |
| Post Translational Modifications | Sumoylated. Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo. |
| Function | Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. |
| Protein Name | Protein HiraTup1-Like Enhancer Of Split Protein 1 |
| Database Links | Reactome: R-HSA-2559584Reactome: R-HSA-9821993 |
| Cellular Localisation | NucleusPml BodyPrimarilyThough Not ExclusivelyLocalized To The NucleusLocalizes To Pml Bodies Immediately Prior To Onset Of Senescence |
| Alternative Antibody Names | Anti-Protein Hira antibodyAnti-Tup1-Like Enhancer Of Split Protein 1 antibodyAnti-HIRA antibodyAnti-DGCR1 antibodyAnti-HIR antibodyAnti-TUPLE1 antibody |
Information sourced from Uniprot.org