• Immunofluorescence analysis of U-2 OS cells using HIBADH antibody (STJ11104297) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using HIBADH antibody (STJ11104297) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using HIBADH antibody (STJ11104297) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Human esophageal using HIBADH Rabbit polyclonal antibody (STJ11104297) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using HIBADH Rabbit polyclonal antibody (STJ11104297) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using HIBADH Rabbit polyclonal antibody (STJ11104297) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HIBADH antibody (37-336) (STJ11104297)

SKU:
STJ11104297

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-3-Hydroxyisobutyrate Dehydrogenase-Mitochondrial (37-336) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HIBADH
Gene ID: 11112
Uniprot ID: 3HIDH_HUMAN
Immunogen Region: 37-336
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-336 of human HIBADH (NP_689953.1).
Immunogen Sequence: ASKTPVGFIGLGNMGNPMAK NLMKHGYPLIIYDVFPDACK EFQDAGEQVVSSPADVAEKA DRIITMLPTSINAIEAYSGA NGILKKVKKGSLLIDSSTID PAVSKELAKEVEKMGAVFMD APVSGGVGAARSGNLTFMVG GVEDEFAAAQELLGCMGSNV VYCGAVGTGQAAKICNNMLL AISMIGTAEAMNLGIRLGLD PKLLAKILNMSSGRCWSSDT YNPVPGVMDGVPSANNYQG
Tissue Specificity Detected in skin fibroblasts.
Protein Name 3-Hydroxyisobutyrate Dehydrogenase - Mitochondrial
Hibadh
Database Links Reactome: R-HSA-70895
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-3-Hydroxyisobutyrate Dehydrogenase - Mitochondrial antibody
Anti-Hibadh antibody
Anti-HIBADH antibody

Information sourced from Uniprot.org

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